lab 1 | detection limits

• (Lower) Limit of Detection: $LOD = \frac{3\sigma}{m}$

• Limit of Quantitation: $LOQ = \frac{10\sigma}{m}$

David Harvey, Analytical Chemistry 2.1 / CC BY-SA

lab 1 | getting started

• You may work in pairs to prepare standards if you wish. But each students should run their own standards/samples individually.
• Create individual lab notebooks.
• Keep glassare clean and organized
  • Wash with Alconox and warm water, 3X DI rinse.
  • All Instrumental I supplies are in back left corner of lab.
• Run a blank (3X) 5 standards (1X each), a QC (3X), and a sample (tonic water, 3X) -- you have 3 weeks for this!
• Your goal today is to get a standard curve with an $R^2 > 0.9990$. If you do not achieve this you should write a short summary of what went wrong in your lab notebook and what you plan to do differently, and then try again (either today or next week).
• You should turn in a draft lab notebook showing either a passing standard curve or a failing standard curve and plans to improve it today on Canvas so I can give you feedback.

lab 1 | getting started

Safety:

• All wastes go in the waste container (in hood).
• Wear gloves and goggles (standards are acidic).
• Ask if you are unsure about anything!

Lab Notebook + Data:

• Turn in notebook at end of lab with today's work
  • You should have blanks, 5 standards, a QC, and (if time) sample data
• You will peer review other notebooks
• You may have to redo the experiment if your results are not good
• Final notebook + (high quality) data due in 3 weeks