CHEM 370 | Week 15

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<h1 style = "text-align: left; font-weight: bold; margin-left: 175px;">Week 15: Chromatography</h1>
<h5 style = "text-align: left; font-weight: bold; margin-left: 175px;">Harvey Ch 12</h5>
<img src="img/chapter-12/column-chromatography.png" style = "margin-left: auto; margin-right: auto; display: block; height: 300px;">

.image-credit[David Harvey / [Analytical Chemistry 2.1](https://chem.libretexts.org/Bookshelves/Analytical_Chemistry/Book%3A_Analytical_Chemistry_2.1_%28Harvey%29) / [CC BY-SA 4.0](https://creativecommons.org/licenses/by-sa/3.0/at/deed.en)]

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# Preparing a Sample

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# Preparing a Sample

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# Chromatography

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# Chromatography

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# Chromatography

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# Chromatography

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# Types of Chromatography

- Planar
- Column

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# Types of Chromatography

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# Types of Chromatography

- Adsorption (liquid-solid)
- Partition (liquid-liquid or gas-liquid)

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# Characterizing Peaks

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# Practice: Retention Factor

A compound elutes with a retention time of 7.21 minutes, and the column's void time is 0.27 min.  What is the retention factor for this compound?

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# Describing a Chromatogram

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# Practice: Resolution

Compound A elutes with $t\_r$ = 8.51 min and $w\_b$ = 0.83 min.  Compound B elutes with $t\_r$ = 9.83 min and $w\_b$ = 0.91 min.  What is the resolution for these two peaks?

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# Characterizing Peaks: Theoretical Plates

.image-credit[H Padleckas / [wikimedia.org](https://commons.wikimedia.org/wiki/File:Continuous_Binary_Fractional_Distillation.PNG) / [CC BY-SA 3.0](https://creativecommons.org/licenses/by-sa/3.0/at/deed.en)]

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# Practice: Theoretical Plates

Compound A elutes with $t\_r$ = 8.51 min and $w\_b$ = 0.83 min.  How many theoretical plates are there?  If the column is 2.0 meters long, what is the height of a theoretical plate in mm?

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# Characterizing Peaks: Asymmetry

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# van Deemter: Eddy Diffusion

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# van Deemter: Longitudinal Diffusion

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# van Deemter: Mass Transfer

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# van Deemter

$$H = A + \frac{B}{u} + Cu$$